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Molecular Devices Corporation. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. More Product Information. Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The chamber. S. Assay Performance with Various Instrument. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 1. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and. HEALTH AND SAFETY CONSIDERAIONSSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Was the failure only of the Detection Estimate (Mag3) Test for the. View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seWe would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax® M5. Table of Contents. 000). Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 72 MB. IMAP technology is based on. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET assays. Published Dec 16, 2020 | Updated Dec 16, 2020. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. cuvette port for assay development • Dual monochromator tunability • Automated absorbance pathlength correction The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dualmonochromator microplate reader. +1 800-635-5577. – ®SpectraMax M4 and M5 Multi-Mode Microplate Readers (cat. 5577. FI assay. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. Measuring cell health on the SpectraMax iD3 reader with cell viability assays. This document is provided to customers who have purchased Molecular Devices, Inc. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. #0200-7011) Instrument setupSoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Turn on Plate Reader and wait. Wide range of applications; Five preferred detection modes;The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. 4. 19. D 1 1. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. SpectraMax M-series Microplate readers. Detection modalities are shown in Table 1-1. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86We would like to show you a description here but the site won’t allow us. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. AquaMax® 2000. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. begin reading microplates with seven mouse clicks. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. ; SoftMax® Pro 7. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Fifteen different automated tests are provided. If values are present, then. Also refer to this Multimode. FLIPR Calcium 6 and 6-QF Assay Kits provide the largest signal window. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Manufacturer: Molecular Devices. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. This problem has been remedied by the introduction of Molecular Devices PathCheck Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. 9. 0 mm wide, and heights from 14 mm to 15 mm. (Molecular Devices Cat. The following document is intended to demonstrate setup of this instrument. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. Why are there four different SoftMax Pro software protocols supplied with the LM1 Plate? 1. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. When the user applies a set temperature of 37°C, the top is heated to 37. Choose cables that meet the requirement described in this appendix. Select Update Driver. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The SpectraMax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and. Lift up the lid over the chamber to insert or remove a cuvette. Read More. -43. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. D. Table of Contents. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Instrument settings used with the SpectraMax M5 Microplate Reader. Background fluorescence has been subtracted. Cavicide (Warehouse, Cat # 79300360) 6. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. 00 +$250. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Homogeneous Time-Resolved Fluorescence (HTRF) . SpectraMax Gemini EM & FLEXstation (bottom read) 1. Molecular Devices Spectramax M 5 Manual, 2000 Vw Beetle Workshop Manual, Proton Supplier Quality Manual, Beltronics Rx65 Red Manual, Vfr 400 Owners Manual, Hgv Mot Testing Manual, Prostart Manual Installation. Molecular Devices SpectraMax® series microplate readers. Molecular Devices SpectraMax M5 Plate Reader Repair. The chamber contains springs that position the cuvette in the proper alignment for a read. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. The M5 has two PMTs. 72 MB. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Optimal instrument settings are the same for all three readers. Cells were treated with increasing concentrations of the antagonist atropine and assayed for response to addition of an EC80 concentration of the agonist carbachol. Spectranet customer portal. 6. Bottom-reading is usually recommended for cell-based assay with adherent cells. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. and California) iv SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Histamine H1 is an endogenous receptor in HeLa cells. Supplier: Molecular Devices. Molecular Devices. This reader's dual monochromators for. Supplier: Molecular Devices. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The following document is intended to demonstrate setup of this instrument and provide representativeMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. To evaluate the amount of miR-148b mimic released by the retro Diels-Alder reaction (Fig. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. If no sound is heard during initialization, contact technical support. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. ] Example of document to evaluate if the reader . Bookings are free, but you will need to use iLab to book. To support these assays, we provide application notes as well as. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Table of Contents. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax M5 Laboratory Equipment. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. 747. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 2. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. 100 readings per well gives optimal Z’ values for this assay. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Fluorescence Microplate Reader Comparison Chart. After running the LM1 Plate protocol, not all values in the values in Results sections are displayed; what should I do? This is a SoftMax Pro software problem rather than anything specific to the LM1 plate, protocol or reader. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi. With a wavelengthon the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Spectranet customer portalMolecular Devices provides a means of validating the optical performance of the SpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. This document is provided to customers who have purchased Molecular Devices, Inc. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. #R7309) 。标记FAM-p34cdc2来源的磷酸化多肽 校正试剂(Molecular Devices Cat. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Published Dec 16, 2020 | Updated Dec 16, 2020. Sale. Find regional support. SoftMax Pro Software can collect data from one or more microplates orSpectraMax ABS Plus SpectraTest ABS2 v1. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. 303 unable to cal dark current. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. 2), the fluorescence intensity of the FAM conjugated to the miR-148b mimic was measured in each well at 495/520 nm (Excitation/Emission) using a Molecular Devices Spectramax M5 Microplate/Cuvette Reader. Our absorbance plate readers feature our PathCheck Sensor. Future ready The SpectraMax i3 x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Turn on the microplate reader. Molecular Devices’SpectraMax ® Microplate Readers have been validated for Transcreener ADP. The SpectraMax M5e Reader offers the additional. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Published Dec 16, 2020 | Updated Dec 16, 2020. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. D. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. D. It has patented optimization, IQ/OQ/PM services, validation tools, and compliance software. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Delivers single mode reader performance in one multimode reader package. This document is provided to customers who have purchased Molecular Devices, Inc. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Failed to load PDF file. 标准功. Products (33). temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. New drivers take 6 days to write, existing drivers take 2 days to. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax Spectrofluorometers. . HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. 2. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. StakMax Microplate Handling System. Table of Contents. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 2. Molecular Devices Spectramax M 5 Manual -. 000). A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. spr SpectraMax Plus 384. Our absorbance plate readers feature our PathCheck Sensor technology and. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Prepare your iD series instrument for shipment. item 6 Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair $8,000. 01 0. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. ®The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. The colorimetric signal development was stopped after 10 minutes. View the Molecular Devices website. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Offer Ends In: 18 Days 19: 38: 21. You should get comparable results from top and bottom reads using EC50's to compare reads. 3: The cuvette chamber. D 1. com A. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. UV/Vis Photometric Performance: Wavelength Range. The StakMax system is an easy-to-set-up, powerful, and reliable stacking solution for. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. The lower limit of detection (LLD) was defined as the level of luciferase detectable above 3 positive standard deviations of the blank. Enzyme dilution curves were performed for Lck, a tyrosine kinase withThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. Fully optimized assays for SpectraMax iD3 reader save time on assay development. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 302 low ref light. Note: In this user guide, all references to SpectraMax Multi-Mode. -65. HEALTH AND SAFETY CONSIDERAIONS The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. -49. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. measure the depth (optical pathlength) of samples with no temperature. View the Molecular Devices websiteThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 4. OPTICSon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax ® M2 Multi-Mode Microplate Reader. Check our website for a current list-ing of worldwide distributors. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. ATP standard curves read on SpectraMax microplate readers. Table of Contents. Learn how SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for Molecular Devices' microplate. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Order Now. S. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the. Prepare your i3/i3x instrument for shipment. -54. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax® M5. Fluorescence Microplate Reader Comparison Chart. View the Molecular Devices websiteMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. 0200-0729. 306 plate air cal fail,low light. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Configurable readers with triple-mode cuvette ports, validation tools, and compliance softwareProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. S. 2. 3. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. ©2022 Molecular Devices, LLC. es •. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. 9000-0756. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Wait 20 seconds. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The CellTiter-Glo assay from Promega uses luciferase. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. #6590-0128) • Set of 2 Fluorescence Polarization Filters 535nm BW 25nm Polarized Vertical & Horizontal (Molecular Devices cat. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Lower end of DNA standard curve. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Schuler measurements were recorded with a Cary UV-Vis Bio100 dual beam spectrophotometer or Molecular Devices SpectraMax M-series. Figure 3. To update the driver. There should be a value for 900 and 1000. These robust readers have been placed in labs from Antarctica to. Preconfigured protocols in SoftMax Pro Software expedite data. 3. Multimode Readers Comparison Chart. Molecular Devices has collaborated with various assay partners to optimize andFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 01 Average RFU DNA Standard (ng. Detection modalities include absorbance (UV-Vis Abs)The chart below provides general information about the availability of on-site and depot repair options. Instrument. SpectraMax DuoLuc Reporter Assay (SMP 6. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. -36. system setup and calibration are controlled from within softmax ro psoftware. We also offer depot Repair Service from our base of operations in Southern California (San Diego). Specifications subject to change without notice. 2018. FP assay do not perform well with protein:protein interactions. On-site Repair Service available nationwide for Molecular Devices SpectraMax M5 Plate Reader's. Lck kinase assay with TAMRA-labeled peptide on. It has a cuvette port, spectral scanning, and up to six wavelengths per read. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. 5. Turn the reader on and secure the USB cable. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 2. If no values are present, the water constant values are missing from the NVRAM. It has. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. MOLECULAR DEVICES SpectraMax M5 is an advanced multi-mode microplate reader which combines fluorescence intensity, fluorescence polarization, luminescence, absorbance and time-resolved fluorescence measurements into one integrated system. 40266 Email: [email protected] adapter for SoftMax Pro and SpectraMax readers. +1 800-635-5577. 2. 1. For top. 3 mm. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. These robust readers have been placed in labs from. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 00 USD. The donor and acceptor can be used to label a variety of biomolecules, for applications. Molecular. Optimal instrument. Additional information is also available in the User Manuals. D 1. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11. #0200-7009The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. SpectraMax Plus 384 measuring instruments pdf manual download. Molecular Devices StakMax® Microplate Stacker within . The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. A highly sensitive instrument with reduced noise and low cross talk, it is also a configurable system with up to 6 channels (photomultiplier tubes) and 12 injectors. Principle of the HTRF IP-One competitive binding assay. Wavelength Range. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 3 or greater. Simple, homogeneous workflow minimizes cell handling. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. g. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 315 can't find zero order. Find regional support. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. handling system from molecular devices. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. 0. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. book Page 1 Wednesday, July 7, 2010 2:09 PMon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Unplug the power cable from the back of the instrument. The following document is intended to demonstrate setup of this instrument and provide representativeThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 72 MB. New applications in minutes Adding modes and functionality is just minutes. The SpectraMax M5 Multi-Mode Microplate Reader is manufactured by Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Assay results are similar on the SpectraMax M5 or other Molecular Devices readers with fluorescence detection mode. This integrated system provides users with a multi. Materials. It has patented optimization, IQ/OQ/PM services,. Molecular Devices Spectramax M5 Microplate Reader Condition: Used, tested in working condition. 1. LoadingIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput. 3. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 1. 5. minutes and begin reading microplates with seven mouse clicks. solution for validating the performance of the SpectraMax 190 .